A novel single nucleotide polymorphism (SNP) of the &amp;lt;i&amp;gt;IGF1R&amp;lt;/i&amp;gt; gene and the association with growth traits in yak (Brief Report)

C Liang,X Guo,M Chu,Y Zeng,P Yan,P Bao,J Pei,Y Yao

doi:10.5194/aab-53-626-2010

Abstract

Abstract. The insulin-like growth factor-I (IGF1) is a peptide growth factor that exerts mitogenic and metabolic activities, which are regulators of growth, survival and cell differentiation in a number of cell and tissue types. To elicit its effects, IGF1 must bind its receptors. The insulinlike growth factor 1 receptor (IGF1R) is similar to insulin receptor (INSR) and it mediates the growth-promoting effect of IGF1. The IGF1R gene, therefore, was selected as a biological candidate gene for growth, body composition, metabolic, and skeletal traits in animals (Rothschild et al. 1997). By now, no polymorphism of yak (Bos grunniensL) IGF1R gene is reported. In the present paper, the partial exon 1 region of IGF1R was screened to detect the SNPs in Chinese yak breeds. Associations of SNP of IGF1R with growth traits were analysed.

Highlights

The insulin-like growth factor-I (IGF1) is a peptide growth factor that exerts mitogenic and metabolic activities, which are regulators of growth, survival and cell differentiation in a number of cell and tissue types
Primer sequences As any of yak insulinlike growth factor 1 receptor (IGF1R) gene sequences was not available, the primer pairs were designed based on bovine partial IGF1R gene
Polymorphism of IGF1R was detected by SSCP in 12 % PAGE in constant voltage (200V) for 12-15 h after its PCR products was denatured 10 min at 98 °C

Summary

Background

The insulin-like growth factor-I (IGF1) is a peptide growth factor that exerts mitogenic and metabolic activities, which are regulators of growth, survival and cell differentiation in a number of cell and tissue types. The insulinlike growth factor 1 receptor (IGF1R) is similar to insulin receptor (INSR) and it mediates the growth-promoting effect of IGF1. The IGF1R gene, was selected as a biological candidate gene for growth, body composition, metabolic, and skeletal traits in animals (Rothschild et al 1997). No polymorphism of yak (Bos grunniens) IGF1R gene is reported. The partial exon 1 region of IGF1R was screened to detect the SNPs in Chinese yak breeds. Associations of SNP of IGF1R with growth traits were analysed

Procedure

Result