Abstract
Objective: A simple, accurate, precise and robust reverse phase high performance liquid chromatography (RP-HPLC) method was developed for simultaneous estimation of ferulic acid, quercetin, piperine and thymol in marketed ayurvedic formulation.Methods: The selected markers were resolved using shim pack GIST C-18 column, with mobile phase acetonitrile: 0.04 M potassium dihydrogen ortho phosphate buffer (pH 3.0 adjusted with ortho phosphoric acid) in a ratio of 60:40 v/v at a flow rate of 1.0 ml/min. The detection was carried out at 264 nm.Results: The retention time of ferulic acid, quercetin, piperine and thymol were found to be 2.98, 3.35, 7.83 and 9.72 min respectively. The developed method was validated according to the guidelines provided in ICH Q2 (R1) in term of linearity, precision, limit of detection, limit of quantification, accuracy and robustness. Linear response for all selected markers was obtained in the concentration range of 12-28 µg/ml with a correlation coefficient (r2) greater than 0.999. The mean % recovery was found to be 99.30 for ferulic acid, 98.77 for quercetin, 100.93 for piperine and 100.25 for thymol.Conclusion: The developed method was applied for quantification of these marker in marketed ayurvedic formulation. This method can be used to evaluate other formulations containing these selected phytoconstituent, thus conforming the quality and safety of ayurvedic or polyherbal formulations.
Highlights
Herbal drugs are used extensively in traditional folk medicine in developed as well as developing countries
The present study focuses on standardization of an Ayurvedic churna formulation using high performance liquid chromatography
All reagents used in this assay were of high performance liquid chromatography (HPLC) grade purchased from thomas baker
Summary
Herbal drugs are used extensively in traditional folk medicine in developed as well as developing countries. Active compounds from herbal sources have always been a great interest for scientists working on infectious and non-infectious diseases. Ayurvedic medicines are polyherbal formulations and every herb consists of an array of chemical constituents. Each ayurvedic formulation is a source of many different phytochemicals, which individuals may have different medicinal properties [1]. The present study focuses on standardization of an Ayurvedic churna formulation using high performance liquid chromatography. The formulation is indicated mainly against intestinal gas, abdominal gas, pain, cough, ulcer, candidiasis, diarrhea, hyperlipidemia, cancer and microbial infection [2]
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