Abstract
In this study, lipid content of live Eriocheir sinensis has been quickly and accurately determined by low‐field 1H Nuclear magnetic resonance (LF‐1H NMR). The experimental parameters of LF‐1H NMR have been optimized and the validity of the established standard method has been confirmed with traditional Soxhlet extraction method. Results show that the lipid signal intensity is strongly correlated with its content and exhibits a good linear correlation (Y = 0.0376 + 4.899X, R 2 = 0.9999), thus demonstrating favorable accuracy and sensitivity for the quantitative determination of lipid content. In conclusion, the lipid content of live E. Sinensis can be directly obtained based on an established method, indicating a great application potential in food and other fields.
Highlights
Chinese mitten crab (Eriocheir sinensis) is an economically important type in China, and has existed for the last few decades
The objectives of this study are to establish a standard method for the quantitative determination of lipid content in live E. sinensis, and to verify the accuracy of LF-1H NMR relative to the Soxhlet extraction method
This study provides a new procedure for the quick determination of lipid content of live E. sinensis
Summary
Chinese mitten crab (Eriocheir sinensis) is an economically important type in China, and has existed for the last few decades. Due to their advanced hatchery techniques and high market demands, the total production of E. sinensis has reached up to 820,000 tons with a market value of 6 billion US dollars in 2015 (China Fishery Yearbook, 2015). The mixtures of liver and gonad tissues (crab roe) of E. sinensis are favorite raw food materials in Asian countries because of their excellent taste and high nutritional value (Jiang et al, 2009; Wang et al, 2016). A previous study has reported that the ovarian lipid concentration reached up to 19.1% in mature stage
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