Abstract
More than 700 microRNAs have been cloned, and the functions of these molecules in developmental timing, cell proliferation, and cancer have been investigated widely. In this report, we describe a novel RT-PCR method for the quantitative analysis of microRNA expression. The use of this method in a model study revealed differential expression of miR-1 and -124 in mouse organs. Specifically, the new method revealed that miR-124 concentrations in the mouse CNS (central nervous system) were more than 100 times those in other organs. By contrast, miR-1 expression in the CNS was 100 – 1,000 times lower than that in skeletal muscle and heart. Moreover, the new method revealed regional differences in miR-124 expression within the CNS: expression ratios versus the cerebral cortex were 60.7% for the cerebellum and 35.4% for the spinal cord. These results suggest that our method would be a powerful new tool for studies of microRNA expression.
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