A novel role of Eruca sativa Mill. (rocket) extract: antiplatelet (NF-κB inhibition) and antithrombotic activities.

Eduardo Fuentes,Iván Palomo,Marcelo Alarcón,Gilda Carrasco,Manuel Fuentes

doi:10.3390/nu6125839

Eduardo Fuentes, Iván Palomo + Show 3 more

Open Access

https://doi.org/10.3390/nu6125839

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Journal: Nutrients	Publication Date: Dec 12, 2014
Citations: 59	License type: CC BY 4.0

Affiliation: University of Talca, Centro de Estudios Científicos

Abstract

Background: Epidemiological studies have shown the prevention of cardiovascular diseases through the regular consumption of vegetables. Eruca sativa Mill., commonly known as rocket, is a leafy vegetable that has anti-inflammatory activity. However, its antiplatelet and antithrombotic activities have not been described. Methods: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL), was evaluated on human platelets: (i) P-selectin expression by flow cytometry; (ii) platelet aggregation induced by ADP, collagen and arachidonic acid; (iii) IL-1β, TGF-β1, CCL5 and thromboxane B2 release; and (iv) activation of NF-κB and PKA by western blot. Furthermore, (v) antithrombotic activity (200 mg/kg) and (vi) bleeding time in murine models were evaluated. Results: Eruca sativa Mill. aqueous extract (0.1 to 1 mg/mL) inhibited P-selectin expression and platelet aggregation induced by ADP. The release of platelet inflammatory mediators (IL-1β, TGF-β1, CCL5 and thromboxane B2) induced by ADP was inhibited by Eruca sativa Mill. aqueous extract. Furthermore, Eruca sativa Mill. aqueous extract inhibited NF-κB activation. Finally, in murine models, Eruca sativa Mill. aqueous extract showed significant antithrombotic activity and a slight effect on bleeding time. Conclusion: Eruca sativa Mill. presents antiplatelet and antithrombotic activity.

Highlights

Found in the Mediterranean diet, the rocket species is from the family, Brassicaceae
The effect of Eruca sativa Mill. extract on P-selectin expression in human platelets after stimulation by adenosine diphosphate (ADP) in PRP was measured by flow cytometry (Figure 1)
(* p < 0.05) denotes a statistically significant difference when Eruca sativa Mill. extract was compared with the activated control analyzed by one-way ANOVA and Tukey’s post hoc test

Summary

Introduction

Found in the Mediterranean diet, the rocket species is from the family, Brassicaceae. The positive and beneficial effects of the phytochemicals contained in rocket on human health have been reported by several clinical research studies. The consumption of green leafy vegetables has been associated with a reduced risk of cardiovascular diseases (CVD) [2]. These beneficial effects have been attributed to the range of phytochemicals they contain, including vitamins A and C, flavonoids and glucosinolates, all of which are found in high levels in Brassicaceae crops [3,4]. The anti-ulcer effect is possibly due to prostaglandin-mediated activity and/or through anti-secretory and antioxidant properties [7,8]. Eruca sativa Mill., commonly known as rocket, is a leafy vegetable that has anti-inflammatory activity.

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