A novel rodent Chapparvovirus in feces of wild rats.

Shixing Yang,Wang Li,Xingli Fu,Yan Wang,Xiaochun Wang,Wen Zhang,Yuan Lin,Zhijian Liu,Quan Shen,Hua Wang

doi:10.1186/s12985-016-0589-0

Shixing Yang, Wang Li + Show 8 more

Open Access

https://doi.org/10.1186/s12985-016-0589-0

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Abstract

Chapparvovirus, a recently determined new genus in the family Parvoviridae, can infect many species of animals including bats, chickens, and pigs. Here, using viral metagenomics method, we identified a novel Chapparvovirus from feces of wild rats and designated it as rat parvovirus 2 (RPV2). The nearly complete genome of RPV2 is 4222-nt long and includes two ORFs encoding a 654-aa nonstructural protein 1 (NS1) and a 472-aa capsid protein (VP), respectively. Phylogenetic analysis over the amino acid sequence of the NS1 showed that RPV2 clustered with Eidolon helvum parvovirus 2 (EHPV2), porcine parvovirus 7 (PPV7), and turkey parvovirus 1 (TP1), forming a separate clade. Sequence analysis indicated that the NS1 protein of RPV2 shared the highest amino acid sequence identity (51 %) with that of EHPV2. According to the genetic distance-based criteria, RPV2 identified here belongs to a novel species of Chapparvovirus.

Highlights

Chapparvovirus, a recently determined new genus in the family Parvoviridae, can infect many species of animals including bats, chickens, and pigs
Sequence analysis indicated that rat parvovirus 2 (RPV2) nonstructural protein 1 (NS1) shares the highest amino acid sequence identity of 51 % with Eidolon helvum parvovirus 2 (EHPV2)
The conserve phospholipase A2 (PLA2) motif widely presented in the other members of the subfamily Parvoviridae is lack in RPV2 capsid protein (VP) protein [11]

Summary

Introduction

Chapparvovirus, a recently determined new genus in the family Parvoviridae, can infect many species of animals including bats, chickens, and pigs. Using viral metagenomics and PCR amplification, we identified a novel species of Chapparvovirus from the feces of wild rats and characterized its nearly complete genome. Viral metagenomics method was used to characterize viral sequences in the fecal samples. B Phylogenetic tree was constructed over the NS1 amino acid sequences of 48 members in the Parvovirinae.

Results

Conclusion