A novel rhodamine-based fluorescent probe for selective detection of ClO– and its application in living cell imaging

Abstract

A novel fluorescent rhodamine-based probe L for selective responding to ClO– has been synthesized and characterized. The spectroscopy showed that probe L can detect ClO– in aqueous solution without interaction with other interfering ions, and the detection is also evident by the colour change from colourless to reddish purple under white light. The remarkable fluorescence enhancement showed the high selectivity and sensitivity of probe L for the detection of ClO–. Furthermore, probe L was applied to intracellular fluorescent imaging of HeLa cells treated with ClO– and MTT assay showed nontoxicity in living cells.