A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin

Mustafa Tunalı,Hakan Özdemir,Emre Yaprak,Zafer Küçükodacı,Serhan Akman,Hülya Toker,Erhan Fıratlı

doi:10.1155/2014/209548

Abstract

We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF). The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun's leukocyte- and platelet-rich fibrin (L-PRF) method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with L-PRF. Blood samples were collected from 10 healthy male volunteers. The blood samples were drawn using a syringe. Nine milliliters was transferred to a dry glass tube, and 9 mL was transferred to a titanium tube. Half of each clot (i.e., the blood that was clotted using T-PRF or L-PRF) was processed with a scanning electron microscope (SEM). The other half of each clot was processed for fluorescence microscopy analysis and light microscopy analysis. The T-PRF samples seemed to have a highly organized network with continuous integrity compared to the other L-PRF samples. Histomorphometric analysis showed that T-PRF fibrin network covers larger area than L-PRF fibrin network; also fibrin seemed thicker in the T-PRF samples. This is the first human study to define T-PRF as an autogenous leukocyte- and platelet-rich fibrin product. The platelet activation by titanium seems to offer some high characteristics to T-PRF.

Highlights

In recent years, there has been a growing interest in the use of platelet-rich products for the treatment of many clinical conditions in dentistry
The blood samples were drawn from the antecubital vein of the subject’s right or left arm in one attempt
Under a higher magnification of the same samples (×400 and ×1000 magnification), the titanium-prepared platelet-rich fibrin (T-PRF) samples showed a highly organized network with continuous integrity compared to the PRF samples (Figures 1(b), 1(c), 1(d)–2(c), and 2(d))

Summary

Introduction

There has been a growing interest in the use of platelet-rich products for the treatment of many clinical conditions in dentistry. This technique requires neither anticoagulant nor bovine thrombin (nor any other gelling agent). This platelet-rich fibrin is considered a second generation platelet concentrate [2,3,4,5,6,7]. Most platelets are activated a few minutes after contacting the tube walls, which initiates the coagulation cascade. Fibrinogen is initially concentrated in the upper part of the tube, before the circulating thrombin transforms it into fibrin. A fibrin clot is formed in the middle of the tube, just between the red corpuscles at the bottom and the acellular plasma at the top [4]

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Discussion

Conclusion