A novel peroxiredoxin from the antagonistic endophytic bacterium Enterobacter sp. V1 contributes to cotton resistance against Verticillium dahliae

Abstract

[Aims] To reveal the molecular mechanism of endophytic bacteria in Verticillium wilt-resistant cottons and deeply understand the interaction between soil and plants. [Methods] Active tracking screening was used for the isolation of antagonistic strain. Morphological and molecular analyses were conducted to determine its phenotypic and genotypic characteristics. MALDI-TOF/TOF MS was employed to identify the antifungal substance. Functional verification was performed by gene cloning, expression, knockout and complementation. [Results] Enterobacter sp. V1 was isolated from Verticillium-wilt-resistant cotton Xinhai 15, which showed significantly antagonistic abilities against cotton verticillium wilt. A peroxiredoxin named ProV1 with virulent against V. dahliae was obtained. The gene for the peroxiredoxin ProV1 was cloned, and the nucleotide sequence showed 85.5% similarity with peroxiredoxin C (WP 096152328) reported from E. hormaeche. The purified heterologously expressed ProV1 indicated strong inhibitory activities. Moreover, the suppression of V. dahliae also decreased in proV1 mutant strain compared with wild type strain. The inhibitory activities could be partially restored by a complementation of proV1 gene in the mutant strain. [Conclusion] The results of this study reveal that a novel peroxiredoxin from an endophytic bacterial strain Enterobacter sp. V1 can serve as a virulent factor against fungi V. dahliae, which helps understanding the interaction between endyphytic bacteria and plant pathogenic fungi, and improving the biological control of cotton Verticillium wilt.