Abstract
In this work, we report SPSS synthesis of a new peptide-based fluorescent probe (L) capable of detecting Zn2+ with little interference in 100% aqueous solutions at physiological pH. Furthermore, L showed excellent sensitivity, with a detection limit of 26.77nM. The 2:1 binding ratio between L and Zn2+ was determined using fluorometric titration, Job's plot and ESI-MS analyses. The "off-on-off" type fluorescence change of L was demonstrated by alternately adding Zn2+ and EDTA based on a formation-separation process of the complex, indicating that L could serve as a reversible probe. Moreover, MTT studies demonstrated that L has low biotoxicity, and could be successfully used for detection of Zn2+ and EDTA in live cells.
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More From: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
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