Abstract
A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. By recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. The described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. Using this method 80.3 ± 5.6% of Staphylococcus aureus with a starting concentration of ~107 CFU/mL and 95.4 ± 1.0% of Methicillin-resistant Staphylococcus aureus with starting concentration of ~104 CFU/mL were removed from 5 mL blood in a few hours. This concept was extended to live rats with an induced bloodstream S. aureus infection. A reduction of two orders of magnitude in the bacterial load of the rats was observed within a few hours. The same technique was used to capture a food pathogen, Salmonella typhimurium, with starting concentrations as low as ~100 CFU, from 100 or 250 mL of culture broth within similar timeframes as above. The feasibility for food pathogen testing applications was additionally confirmed by capturing and detecting S. typhimurium in ground chicken and ground beef.
Highlights
A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens
Several tubes with antibodies can be used enabling capture of multiple kinds of pathogens simultaneously. In this manuscript, capturing of microbial pathogens is demonstrated such as S. aureus, MRSA from blood at high concentration in-vitro and in-vivo and S. typhimurium from culture media and food matrices in very low concentrations
Tube capturing for MRSA resulted in an average 95.4 ± 1.0% reduction (n = 5), as summarized in Fig. 2c
Summary
A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. The capture and detection by real time PCR of MRSA in clinically relevant low concentrations was confirmed After 3 hours from the injection, extracorporeal circulation through a 240-cm antibody coated tube was performed for 5 hours at 0.5 mL/min flow rate (Fig. 3a).
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