Abstract
A simple passive sampling protocol using cheesecloth and electronegative filters coupled with a Tween®20-based elution technique provided reliable detection of SARS-CoV-2 in wastewater at targeted locations in a region of low COVID-19 prevalence.
Highlights
This study demonstrated the effectiveness of an innovative 3D-printed passive sampling device with new extraction techniques for SARS-CoV-2 detection in municipal wastewater in a region with low COVID-19 prevalence
cycle threshold (Ct) values for RNA extracted from cheesecloth, gauze, and electronegative filters in DI water ranged from 36.8 to 37.2, 36.8 to 37.5, and 35.6 to 37.3 respectively
Ct values for RNA extracted from cheesecloth, gauze, and electronegative filters in wastewater ranged from 34.6 to 35.2, 35.3 to 35.6, and 34.8 to 35.5 respectively
Summary
This study demonstrated the effectiveness of an innovative 3D-printed passive sampling device with new extraction techniques for SARS-CoV-2 detection in municipal wastewater in a region with low COVID-19 prevalence. A passive sampling technique commonly referred to as the “Moore swab” has been previously used to extract enteric pathogens from cotton gauze in water.[25,26] Recently, the Moore swab approach was used for passive sampling of SARS-CoV-2 in wastewater and it was determined that passive samplers were at least as sensitive as conventional sampling for detecting SARS-CoV-2 in wastewater.[24] Another benefit of the passive sampling approach for WBE is that these devices can be deployed at smaller scales at sewer locations or pump stations for targeted monitoring at a specific location or building where grab or composite sampling may not be feasible. There is a need to comparatively evaluate elution efficacy of the various buffers or mixtures that have been considered in the literature
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