A Novel Paper-plastic Microfluidic Hybrid Chip Integrated with a Lateral Flow Immunoassay for Dengue Nonstructural Protein 1 Antigen Detection

Abstract

This study presents an easy paper-plastic hybrid microfluidic chip fabrication method that explored the use of oven baking a wax-printed pattern for microfluidic channel assembly combined with the use of a double-sided adhesive tape for layer binding. More specifically, the method includes printing of a wax pattern onto the CA film & oven baking of the pattern along with the backed NC membrane at 120°C for 2.5 minutes. The porous nature of the NC membrane allows the wax pattern to enter into its pores during the baking process and binds the backed NC membrane with the CA film. Attachment of the engraved PMMA layer was performed to increase the structural support. The engraved hole of the layer allows the conjugate pad with space for its integration into the system. Moreover, the antibodies required for the integration of the chip with the LFIA were selected by enzyme-linked immunosorbent assay (ELISA). A CA film, an NC membrane, and a PMMA sheet were used to develop a microfluidic chip that can be integrated with a sandwich format lateral flow immunoassay used to detect dengue non-structural 1 protein. The fabricated chip was able to successfully detect a concentration of dengue non-structural 1 protein of at least 84.66 ng/mL.