Abstract
A novel 1-aminocyclopropane-1-carboxylate (ACC) oxidase cDNA clone ( CP-ACO2, 1485 bp) was isolated from papaya for the first time. The deduced amino acid sequence shares 77% identity with the previously reported papaya CP-ACO1. Genomic Southern analysis with gene specific probes showed that CP-ACO2 is single copy in papaya genome. It was also identified to be ethylene and wounding inducible, an observation in agreement with the presence of ERE and WUN cis-acting elements in CP-ACO2 promoter region. Northern blot analysis revealed that CP-ACO2 was induced only at the late stage of fruit ripening and leaf senescence, while CP-ACO1 was induced before color break (mature) stage. These results suggest that CP-ACO2 is late stage associated, occurring during organ senescence, such as fruit ripening and leaf senescence; while AP-ACO1is maturation associated are possible. Therefore, two distinct pathways for ACC oxidase regulated ripening, one is regular ripening associated, the other is late stage ripening associated.
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