Abstract
Authenticity of dried aromatic herbs and herbal powders for the ASU (ayurvedic, siddha, unani) drug formulations is a key of their clinical success. The DNA based authentication is an answer; however, extraction of PCR quality DNA from such material is often problematic due to the presence of various co-extracted PCR inhibitors. Here, we report a novel DNA isolation and purification method utilizing cow skim milk that successfully yields PCR quality DNA from the aromatic herbs and dried herbal powders. The improved method presented in this study could be used as an alternative to successfully extract PCR quality DNA from such plant materials. Further, we present a set of robust matK primers which could be used as plant barcoding resource in future studies.
Highlights
Authenticity of dried aromatic herbs and herbal powders for the ASU drug formulations is a key of their clinical success
Our method is modified from the cetyltrimethylammonium bromide (CTAB) m ethod[16] and the key novel step in this method is the use of cow skim milk (0.1% fat) during the CTAB lysis of dried herbs and aromatic plants
Taking the clue from above studies, we anticipated that the skim milk might help in the extraction of pure DNA from dried aromatic herbs and herbal powders, where it is difficult to extract pure DNA due to the presence various secondary metabolites that inhibit the PCR reactions and hamper the downstream molecular applications
Summary
Authenticity of dried aromatic herbs and herbal powders for the ASU (ayurvedic, siddha, unani) drug formulations is a key of their clinical success. 2,3,4,7 The identity of these specimen vouchers of dried herbs was confirmed by inclusion of reference samples for Adhatoda vasica/Justicia adhatoda[2] (synonyms), Piper longum[3], Psoralea sp./Cullen sp.[4] (synonyms), and Butea monosperma[7], respectively (Reference code CH73, CH10, SV3/J and WB4 respectively in Supplementary Fig. 3).
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