Abstract

Infected wounds are major challenges for hospitalized patients. Phenylethyl alcohol is a plant active compound with antibacterial activity and antioxidant properties that may decrease infection in wound site. This study investigates the effects of topical application of gels prepared from Phenylethyl alcohol (PLL) and its nanoliposomes (PLL/NLPs) for the healing of infected excision full-thickness wounds in mice model. Circular wounds were induced in 72 mice, inoculated with Acinetobacter baumannii and Streptococcus pyogenes bacteria and treated with blank gel (Conl), mupirocin® (Mupn), and therapeutic gels of PLL, and PLL/NLPs. In vitro and in vivo antibacterial activities, antioxidant status in wound site, histopathological parameters, immunofluorescence staining for TNF-α and COL1A rate expressions, and FGF, EGF, COL1A, and NF-κB proteins expression were investigated by Western blot analysis. The results showed significant safety and antibacterial activity of Phenylethyl alcohol and its NLPs. Topical administration of therapeutics gels, especially PLL/NLPs, significantly (P < 0.05) decreased edema rate and inflammation via decreasing TNF-α and NF-κB proteins expressions, improving antioxidant status and increasing the expression of FGF, EGF and COL1A (P < 0.05). The results showed a competition between mupirocin ointment and PLL/NLPs gel. In sum, topical administration of PLL and PLL/NLPs gels accelerated the wound healing process by decreasing the inflammation and moving wound process toward proliferative phase via molecular, pathological, antibacterial and antioxidant mechanisms. PLL/NLPs gel can be used for the treatment of infected excision full-thickness wounds after future clinical studies.

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