Abstract
A novel monoclonal antibody was raised against a syntheticN-myristoyl glycine that is characteristic of allN-myristoylated proteins. The immunoreaction suppressed in the presence of hemocyanin as well as albumin conjugated withN-myristoyl glycine and otherN-myristoyl glycyl peptides, while underivatized and myristoyl amino acid proteins or various fatty acids other than myristic acid exerted no effect. The antibody specifically reacted withN-myristoylated pp60c-srcin human colon adenocarcinoma cells,N-myristoylated pp60v-srcin Rous sarcoma virus-infected cells, andN-myristoylated Gag precursor protein Pr55gagin HIV-1-producing cells. Furthermore, the antibody immunoreacted with a newN-myristoylated p28gagderived from HIV-1gagprotein. The antibody is shown to be a very useful tool for identification ofN-myristoylated proteins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
