Abstract
IL-2 is a pleiotropic cytokine produced by T cell after antigen activation of T cell and it is so called T cell growth factor. A large number of documents suggest that Il-2 plays pivotal roles in the immune response and now Il-2 is an approved drug being used for various kinds of diseases such as cancer and dermatitis.1 The aim of present exploration was to look at effect of IL-2 on structural, phenotypic and functional maturation of murine BMDCs. The structural and phenotypic maturation of BMDCs under influence of IL-2 were evaluated by light microscope and flow cytometry (FCM). The functional maturation of BMDCs was confirmed by cytochemistry assay, FITC-dextran, acid phosphatase (ACP) activity, bio-assay and enzyme linked immunosorbent assay (ELISA).We elucidated that IL-2 up-regulated the expression of key surface markers such as: CD80, CD83, CD86, CD40 and MHC II molecules on BMDCs, down-regulated phagocytosis activity, induced more production of IL-12 and TNF-α secreted by BMDCs. Therefore it can be concluded that IL-2 effectively enhance the maturation of BMDCs. Our results provide direct evidence to support IL-2 would be used as a potent adjuvant in preparation of DC-based vaccines, as well as an immune remedy for cancer situation.
Highlights
IL-2 is an interleukin, a type of cytokine and a soluble signaling molecule mediating in the immune system
The functional maturation of BMDCs was confirmed by cytochemistry assay, FITC-dextran, acid phosphatase (ACP) activity, bio-assay and enzyme linked immunosorbent assay (ELISA).We elucidated that IL-2 up-regulated the expression of key surface markers such as: CD80, CD83, CD86, CD40 and MHC II molecules on BMDCs, down-regulated phagocytosis activity, induced more production of IL-12 and TNF-a secreted by BMDCs
Post treatment with 25mg /ml IL-2 for 48h immature BMDCs developed into maturation confirmed by upregulated expression of key surface markers such as: CD86, CD80, CD83 CD40 and MHC-II, which will work as key signal and co-signals to activate T cell
Summary
IL-2 is an interleukin, a type of cytokine and a soluble signaling molecule mediating in the immune system. IL-2 mediates a series of immunological effects by binding to IL-2 receptors on the surface of lymphocytes[3] and the cellular responses in our body are regulated by the amount of IL-2 produced in response to antigens. It stimulates the production of IL-2 receptors on the surface of immune cells and acts through autocrine or paracrine way. We performed the following research to look at phenotypic and functional effect on DC by IL-2
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