Abstract
BackgroundLiver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). To date, the molecular mechanism regulating the development of liver CSCs remains unknown.MethodsIn this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh7 cells conditioned medium (CM). miPSCs treated with CM were injected into the liver of BALB/c nude mice. The developed tumours were then excised and analysed.ResultsThe primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers.ConclusionsWe established a model of liver CSCs converting from miPS and showed different stages of stemness during conversion process. Our CSC model will be important to assess the molecular mechanisms necessary to develop liver CSCs and could help in defeating liver cancer.
Highlights
Liver cancer is the second most common cause of cancer-related death
The molecular mechanism of the liver cancer development has been studied for many years, these studies focussed only on the cancer cells, which are present in the cancer tissues, but not the origin of these cancer cells, which are known as the liver cancer stem cells (CSCs)
RESULTS mouse induced pluripotent stem cells (miPSCs) survived in the presence of conditioned medium (CM) of hepatocyte-derived carcinoma cell line Huh[7] cells Usually, induced pluripotent stem cells (iPSCs) are considered to be induced progenitor cells, which differentiate into various normal phenotypes, just like embryonic stem cells (ESCs), depending on the normal niche
Summary
Liver cancer is the second most common cause of cancer-related death. Every type of tumours including liver cancer contains cancer stem cells (CSCs). The molecular mechanism regulating the development of liver CSCs remains unknown. METHODS: In this study, we tried to generate a new model of liver CSCs by converting mouse induced pluripotent stem cells (miPSCs) with hepatocellular carcinoma (HCC) cell line Huh[7] cells conditioned medium (CM). RESULTS: The primary cultured cells from the malignant tumour possessed self-renewal capacity, differentiation potential and tumorigenicity in vivo, which were found rich in liver cancer-associated markers as well as CSC markers. With the number of deaths estimated as 746,000 in 2012, liver cancer is the second leading cause of cancer mortality in the world. Liver CSCs are currently considered as a specific subpopulation with significant tumorigenic potential, which should contribute to the development and recurrence of HCC.[4]
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