Abstract
In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO2 fixation. Cell growth under different CO2 supply conditions (0.2, 0.4, 0.8, and 1.2 g L−1 d−1), residual nitrate, and pH value of Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, and Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A sample screen of six microalgal species (Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, Selenastrum capricornutum, Scenedesmus obliquus SJTU-3, Chlamydomonas sajao, Dunaliella primolecta) was carried out in flasks and the M96SS. Chlamydomonas sajao appeared to be a robust performer (highest cell density: 1.437 g L−1) in anaerobic pond water with 0.8, and 1.2 g L−1 d−1 CO2. The reliability and efficiency of the M96SS were verified through a comparison of traditional flask culture, M96SS, Lukavský’s system, and a microplate shaker.
Highlights
Global warming is of great concern [1]
Chlorella pyrenoidosa SJTU-2 was cultivated in each well of two microplates in the M96SS fed with 0.8 g L−1 d−1 CO2
SJTU-3, Chlorella pyrenoidosa SJTU-2, Selenastrum capricornutum, Scenedesmus obliquus SJTU-3, Chlamydomonas sajao, and Dunaliella primolecta) were simultaneously cultivated in flasks and the M96SS under four CO2 supply conditions (0.2, 0.4, 0.8, and 1.2 g L−1 d−1) and three types of liquid medium (Dunaliella primolecta was cultivated in f/2 medium, anaerobic pond water, and oxic pond water, while the other five strains were cultured in BG-11 medium, anaerobic pond water, and oxic pond water)
Summary
Global warming is of great concern [1]. Carbon dioxide (CO2), the principal greenhouse gas, is an important causative factor in global warming. Microalgae are unicellular microorganisms that rapidly fix CO2 via highly efficient photosynthesis They are of great potential to produce considerable amounts of low-carbon-emission biofuels, which could lead a green revolution in the future [2]. Some miniature culture technologies have been used to increase experimental throughput in microalgae research [6,7,8]. Most of these are adaptations of Lukavský’s [9] system. Later researchers [5,10] verified the agreement between Lukavský’s [9] system and the flask culture system for bioassays These authors did not address similarities and differences between microalgal growth characteristics in miniature and flask culture. The comparisons of pH, cell density, and residual nitrate in both systems showed that there was a good agreement between results from the M96SS and standard flask culture methods
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