Abstract
Many photochemical or photobiological applications require the use of high power ultraviolet light sources, such as high-pressure mercury arc lamps. In addition, many photo-induced chemical, biochemical and biological applications require either a combinatorial setting or a parallel assay of multiple samples under the same environmental conditions to ensure reproducibility. To achieve this, alternative, controllable light sources, such as ultraviolet light emitting diodes (UV LEDs) with high power and spatial control are required. Preferably, LEDs are arranged in a suitable standardized 96-well microtiter plate format. We designed such an array and established the methods required for heat management and enabling stable, controllable illumination over time.
Highlights
Light emitting diodes (LEDs) have several advantages compared to more traditional illumination sources: They are more efficient, have a long life time, compact size, and high reliability [1] ModernLEDs allow long-term generation of irradiation of a wide range of wavelengths [2]
The growing importance of LEDs in industry, science and research is best demonstrated by rising numbers of publications; the number of publication about ultraviolet light emitting diodes (UV LEDs) increased by 42 times from 2000 to 2016 [6]
The use of high-power LEDs in research is exemplified by Hölz et al [7], who replaced a commonly used high-pressure mercury arc lamp with an ultra-high power 365 nm LED for use in photolithography, offering economic and ecological advantages, as well as lower hardware costs and a very long lifetime
Summary
Light emitting diodes (LEDs) have several advantages compared to more traditional illumination sources: They are more efficient, have a long life time, compact size, and high reliability [1] Modern. LEDs allow long-term generation of irradiation of a wide range of wavelengths [2]. Recent advances in LED technology provide efficient light sources in the UV and blue wavelength region [3,4,5]. The use of high-power LEDs in research is exemplified by Hölz et al [7], who replaced a commonly used high-pressure mercury arc lamp with an ultra-high power 365 nm LED for use in photolithography, offering economic and ecological advantages, as well as lower hardware costs and a very long lifetime. UV radiation seems to play a role in forming
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