Abstract
Currently the state of the art method for measuring strain in individual muscle myofibrils is to isolate them for attachment to microtools that act as force measuring cantilevers and length controllers. However, this reductionist approach omits the physical and native context that could influence the force generating properties of myofibrils and myocytes, including crowding of other organelles, the microtubular network, and connection to the matrix and other cells via adhesion complexes. To study calcium dependent strain of native myofibril networks in intact human cardiomyocytes, we are developing a system to induce activation and contraction of live human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) through treatment with gentle detergent and buffers of varying calcium concentration.
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