Abstract
Penicillin acylase from Bacillus megaterium was immobilized to the functional groups of the basic anion exchange resin Diaion CR-20 using hexamethylene diisocyanate and p-hydroxybenzaldehyde as the coupling reagents. A specific activity of 135 U/g resin was obtained with an activity yield of 45%. The addition of 0.3 mM Ca2+ improved the stability of the immobilized enzyme, with its remaining activity in a recycling system at the 300th time being about 70% of the initial value.
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