Abstract
An assay for factor-dependent ribosome dissociation has been developed, by coupling with the peptidyltransferase reaction between acylaminoacyl-tRNAs and puromycin. The peptidyltransferase reaction is specific for 60S subunits; it is inhibited by derived 40S subunits which combine with derived 60S subunits to form ribosomes. Native 40S subunits, and derived 40S subunits treated with an extract (IF-3) obtained from native 40S subunits, do not interact with 60S subunits and they do not affect the 60S-dependent peptidyltransferase reaction. The native 40S extract releases subunits from 80S ribosomes and the 60S subunits released, a measure of dissociation, can be determined by the peptidyltransferase reaction.
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