Abstract

A novel non-instrumental method for the primary diagnosis of group A streptococci from clinical specimens notably throat swabs is described. The method is based upon a sandwich immunoassay. The assay format consists of a dot-blotting procedure and immunodiffusion on nitrocellulose of pore sizes 0.45μ and 5μ respectively. The detection reagent consists of specific polyclonal rabbit antibodies covalently bound to colloidal carbon particles.KeywordsThroat SwabSandwich ImmunoassaySpecific Polyclonal AntibodyStreptococcal DiseaseEnzymatic Substrate ReactionThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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