A novel method for the generation of linear structured illumination for structured illumination microscopy

Abstract

We propose a novel method for generating linear structured illumination using phase modulation. We implemented linear structured light using holographic technique. The computer generates double concentric annular slits with different radius, a prism phase is applied on the slits to tilts the beam that incidents on the slits away from the optical axis. Different annular beam produce Bessel beams with different axial wave vectors, linear structured light can be obtained from the interference between two Bessel beams. The period and phase of linear structured light can be adjusted by adjusting the radius and the initial phase difference of the double concentric annular beams. We theoretically and experimentally verify that the method can effectively generate linear structured light.