Abstract
Zinc deficiency is correlated with many pathologies, but the molecular connection between disease states and insufficient zinc is still poorly understood. To investigate the connection between zinc deficiency and organismal phenotypes, we developed a technique to specifically diminish zinc levels in mammalian cell culture media without perturbing the concentrations of other metal ions. Growth of cells in such depleted medium evokes some of the transcriptional responses previously noted in organismal models of zinc deficiency, which can be restored upon addition of zinc to the original levels. RNASeq and miRNA data similarly support a zinc‐specific response to the depletion. We also report how the zinc transcriptome remodels in the context of zinc limiting growth. This robust and transportable methodology offers investigators a unique tool for studying zinc deficiency in the context of mammalian cell culture.Support or Funding InformationThe work was supported by the US National Institutes of Health and the Mallinckrodt Faculty Scholar Award.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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