Abstract
This study presents a novel method for predicting the shelf life of pork in real-time based on front-face fluorescence excitation-emission matrices (EEMs). The total viable count (TVC) of bacteria was used as the indicator of microbial spoilage in the pork samples. Modified Gompertz and square root equations were used to establish models for the trends in microbial growth and for predicting the shelf life, the R2 values of the fitting equation at different temperatures were all greater than 0.95. The fluorescence intensity ratio of oxidation product to tryptophan (FOX/Trp) was highly correlated with the quality deterioration of pork and was therefore used to establish a quantitative model of TVC values by linear regression with Rc2 and Rp2 values of 0.914 and 0.906, respectively. The mean absolute errors between the remaining shelf life predicted by fluorescence EEMs and the measured values at three storage temperatures were less than 1 day.
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