Abstract
We have examined various methods for introducing an engineered fluorescent dye molecule-labeled troponin into cultured primary mouse ventricular myocytes. The troponin complex is transported through the cell membrane using a cell-penetrating peptide and allowed to exchange with endogenous troponin within the myofilament. Exchange has been monitored using scanning confocal imaging and by fluorescence lifetime imaging (FLIM). This work may enable single pair FRET measurements of myocardial activation in live cells.
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