Abstract
AbstractA new kind of MALDI matrix, termed paraffin wax immobilized matrix, was used to study peptide mixtures and proteins. During the preparation process, the paraffin wax was heated and coated on the stainless‐steel target plate, and then 2,5‐dihydrobenzoic acid (DHB) was deposited on the paraffin layer and stainless‐steel target plate to obtain different kinds of matrix spots. The morphology of matrices on different supports and peptide‐matrix co‐crystallization were observed by a high resolution digital‐video microscopy system. Peptide mixtures and bovine serum albumin (BSA) digests were used to investigate the performance of the immobilized matrices on the paraffin target. The MALDI‐FTMS analysis results also showed that the detection sensitivity of matrices immobilized in the paraffin sample support was better than that on other sample supports.
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