A Novel LncRNA, AC091729.7 Promotes Sinonasal Squamous Cell Carcinomas Proliferation and Invasion Through Binding SRSF2.

Boyu Yu,Linli Tian,Tianyi Wu,Yanan Sun,Ming Liu,Qiuying Li,Like Yang,Bingrui Yan,Yushan Li,Linmei Qu,Xuan Kan,Xuehui Zhao

doi:10.3389/fonc.2019.01575

Abstract

Long non-coding RNAs (lncRNAs) play important roles in various biological progresses of carcinogenesis. However, the function of lncRNAs in human sinonasal squamous cell carcinoma (SNSCC) remains greatly unclear. In the current study, lncRNA AC091729.7 expression was examined in SNSCC samples by using microarray, RNA in situ hybridization (ISH) and real-time fluorescence quantitative PCR (qRT-PCR). Cell viability, colony-formation, wound-healing, and transwell assays were applied to SNSCC cells. Xenograft mouse models were employed to evaluate the role of AC091729.7 in growth of SNSCC in vivo. Human protein microarray (HuprotTM Protoarray) and RNA immunoprecipitation (RIP) were used for identifying AC091729.7 binding proteins in SNSCC. Results showed AC091729.7 was upregulated and closely connected with the survival of the SNSCC patients. Knockdown of AC091729.7 suppressed SNSCC cell migration, proliferation, invasion in vitro. Furthermore, downregulation of AC091729.7 could inhibit the growth of SNSCC in vivo. Moreover, Human protein microarray and RIP suggested that AC091729.7 directly combine with the serine/arginine rich splicing factor 2 (SRSF2). Our results suggest that in the cell progression of SNSCC, lncRNA AC091729.7 plays a carcinogenic role and serves as a novel biomarker and latent curative target in SNSCC patients.

Highlights

Nasal cavity and paranasal sinus malignancies account for 3% of those in the head and neck region and 1% of the total [1]
We evaluated the correlation between AC091729.7 expression and the clinicopathological parameters in 60 sinonasal squamous cell carcinoma (SNSCC) patients
A previous study reported that high expression of TrkB plays a major role in SNSCC, and TrkB can be used as a potential prognostic indicator for the clinical prognosis [25]

Summary

Introduction

Nasal cavity and paranasal sinus malignancies account for 3% of those in the head and neck region and 1% of the total [1]. Squamous cell carcinoma (SCC) is the most common histological type of nasal malignancies [2]. Sinonasal squamous cell carcinomas (SNSCC) tend to occur in advanced stages, accompanied by local damage and unfavorable prognosis. A number of studies have showed diagnostic and prognostic values of lncRNAs in in head and neck squamous cell carcinoma. Such as long non-coding MIR205HG could cause unlimited proliferation of head and neck squamous cell carcinoma [18]. Long Non-coding RNA FAM225A promotes Nasopharyngeal Carcinoma tumorigenesis and metastasis [19]. LncRNA SSTR5AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma [20]. LncRNA-p23154 promotes the invasionmetastasis potential of oral squamous cell carcinoma [21]. A novel mechanism showed that lncRNA regulates the pathogenesis of SNSCC

Methods

Results

Conclusion