A novel l-arabinose isomerase from Lactobacillus fermentum CGMCC2921 for d-tagatose production: Gene cloning, purification and characterization

Abstract

Abstract The araA gene encoding l -arabinose isomerase ( l -AI) from the acidophilus bacterium Lactobacillus fermentum CGMCC2921 was cloned and over-expressed in Escherichia coli. The open reading frame of the l -AI consisted of 1425 nucleotides encoding 474 amino acid residues. The molecular mass of the enzyme was estimated to be approximately 53 kDa on SDS–PAGE. The purified recombinant enzyme showed maximum activity at 65 °C and pH 6.5, which were extremely suitable for industrial applications. It required divalent metal ions, either Mn2+ or Co2+, for enzymatic activity and thermostability improvement at higher temperatures. The enzyme was active and stable at acidic pH, it exhibited 83% of its maximal activity at pH 6.0 and retained 88% of the original activity after incubation at pH 6.0 for 24 h. Kinetic parameter study showed that the catalytic efficiency was relatively high, with a kcat/Km of 9.02 mM−1 min−1 for d -galactose. The purified L. fermentum CGMCC2921 l -AI converted d -galactose into d -tagatose with a high conversion rate of 55% with 1 mM Mn2+ after 12 h at 65 °C, suggesting its excellent potential in d -tagatose production.