Abstract
l-Cysteine (Cys) and chitosan (Chi) were cross-linked to form the functional polymer composite with active groups of thiol and amino groups (denoted as Chi-Cys) under the coupling reagents of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydrosuccinimide (EDC/NHS). Then it was modified on the surface of glassy carbon electrode (GCE). The active groups in Chi-Cys composite can adsorb gold nanoparticles by self-assembly technique. The electrochemical immunosensor can be fabricated by using gold nanoparticles-functional chitosan biological composite film as matrix to immobilize the monoclonal antibody against semicarbazide (SEM) (SEM-McAb). The detection of SEM residue was performed with the technique of electrochemical impedance spectroscopy. Under the optimized conditions, the relative change in impedance was proportional to the logarithmic value of SEM concentrations in the range of 1.0–1.0×104ngmL−1 (r=0.9998) with the detection limit of 1.0ngmL−1. The specificity, reproducibility, stability, and accuracy of the proposed impedimetric immunosensor were also evaluated. The proposed immunosensor showed economical, efficient, and potential application for the detection of SEM in food samples.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.