A novel investigation on characterization of bioactive glass cement and chitosan-gelatin membrane for jawbone tissue engineering

Abstract

The aim of this work was to investigate the characterization of a bioactive glass (BG) ceramic prepared by sol–gel technique and chitosan-gelatin membranes prepared by lyophilization technique containing 0, 1 and 2 vol.% ethanol. Early-absorbing and late-absorbing bone substitutes in practice constitute the main volume of bone substitutes used by dentist and orthopedic surgeons. When the graft is to be gradually replaced by normal bone, the important issue is the duration and rate of graft absorption. The prepared samples were characterized using fourier transformed infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis. The presence of Ag nanoparticles into bioactive glass was evaluated. Porous membranes were examined under scanning electron microscopy (SEM) to estimate the size of the pores and analysis the morphological behavior. The pore diameter could be controlled within the range 10–30 µm by adjusting the percent of ethanol. The formation of needle-like hydroxyapatite (HA) crystals on the surface of the membrane after 7 days immersion in the ringer's solution was also assessed using SEM images. The SEM images results illustrated the porous structures in the membranes. The average pore size for chitosan-gelatin membrane with 2% ethanol were 30±6.3 μm. Finally, these obtained results suggested that the developed membrane with 2% ethanol possess the prerequisites for tissue engineering and can be used for jawbone tissue engineering applications.