Abstract
In this work, a high-precision visual and point-of-care testing (POCT) ratiometric fluorescent nanosensor for TC was constructed by embedding fluorescein (FL) into l-histidine modified ZIF-8 (LZIF-8) and chelating with rare earth citrate complex. Upon exposure to TC, the coordination and energy transfer between Eu3+ and TC make Eu3+ sensitized emission characteristic red fluorescence, while without prejudice to the emission peak intensity of FL. This ratiometric fluorescent probe with stable reference signal had the characteristics of high sensitivity (ultralow LOD = 5.99 nM) and selectivity and fast response kinetics (within 20 s). The high-bright fluorescence emission was beneficial to human eye recognition and it can realize semi-quantitative detection of TC in eggs, meeting the requirements of household use. In addition, the color can be read and analyzed quickly with the help of smart phone color analysis APP, and the semi-quantitative concentration of TC can be determined more conveniently.
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