Abstract

A new probe 4-bromo-2-((E)-((Z)-((5-bromo-1H-indol-2-yl) methylene) hydrazono) methyl) phenol (BHPL) has been developed and successfully employed for the detection of Al3+/Zn2+/F-ions in DMSO: H2O (9:1) system. The photophysical evaluation of BHPL with cations/anions were rationalized by UV–visible and fluorescence spectroscopy. The stoichiometric binding mode of BHPL with Al3+/Zn2+/F- ions were confirmed by Job’s plot analysis and strongly supported by1H NMR titration,19F NMR analysis and electrospray ionization mass spectroscopy (ESI-MS) techniques. The optimized geometries of BHPL with their complexes and ground state energy levels were gauged via DFT calculations. In the biological environment, BHPL could perform as an obvious fluorescent chemical tool to detect Zn2+ionsin HeLa cells and fluorescence imaging analysis in Zebrafish embryos.

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