Abstract
A serological kit was prepared for the first time to detect bacteria that produce Gramicidin (S). Since, an immunocomplex of antibiotic Gramicidin (S) was prepared followed by immunonization of laboratory animales to obtain specific antibodies in their serum. Serial dilutions of the prepared antibodies were tested directly against samples of bacteria and compared with control samples, which include bacteria that produce Gramicidin (S) (as a positive control) and the bacteria that does not produce Gramicidin (S) (as a negative control) depending slide method after the incubation of bacteria in a suitable media for 3 - 4 hours at 37°C. The prepared kites were evaluated in reference Laboratories and they proved to be highly sensitive (100%), specific (100%), more economic and reliable procedure, in addition to its stability for more than one year. It was the first time to prepare a kit for detection of bacteria which produce Gramicidin (S) directly within few minutes.
Highlights
Gramicidin (S) (Scheme 1) has historically been employed as a topical antibiotic for the treatment of infections from superficial wounds
1) Cellulose acetate paper electrophoresis: Test was done by using cellulose acetate paper electrophoresis in Educational Medical City laboratories [9]
Results of the analysis forms three separated different bands, the Bovin Serum Albomin (BSA) band had cleared the site and the status of normal, while the synthesized immune complex (BSA bond with Gramicidin (S)) at the site before with a wider area than albumin band, whatever the same concentration of both free and bond BSA had been used in the experiment and the method adopted was cellulose acetated paper electrophoresis [6,13]
Summary
Gramicidin (S) (Scheme 1) has historically been employed as a topical antibiotic for the treatment of infections from superficial wounds It exhibits strong antibiotic activity against a broad spectrum of Gram negative, Gram-positive bacteria and against several pathogenic fungi [1]. Gramicidin (S) produced by the Gram positive bacterium Bacillus brevis, it is a cyclodecapeptide, constructed as two identical penta peptides joined head to tail, formally written as cycle (-Val-Orn-Leu-D-Phe-Pro-)2 [2]. It forms a ring structure composed of five different amino acids, each one used twice within the structure [3]. The purpose of this research was to find a direct immunological technique characterized with sensitivity and specificity for the detection of antibiotic producing bacteria in short period
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