Abstract
Systemic lupus erythematosus (SLE) is a complex multi-system autoimmune disease. Detection of anti-nuclear antibodies (ANA) is fundamental for the diagnosis of SLE. In the present study, we found that the level of core fucosylation catalyzed by α1,6-fucosyltransferase (Fut8) is markedly up-regulated on immunoglobulin G (IgG) in the sera of SLE patients detected by Aspergillus oryzae lectin (AOL) blot. In sandwich Dot enzyme-linked immunosorbent assay (Dot-ELISA), the core fucosylation level was also found significantly increased in the sera from SLE patients with a higher ANA titer. To establish a rapid and sensitive laboratory test for the diagnosis of SLE, we prokaryotically expressed AOL and C3-D1-C3-D2-C3 of protein G (SpG3), and generate AOL-conjugated colloid gold immunochromatographic strips (ICS). The detection limit of core fucosylated IgG was 10 μg/mL for AOL-conjugated colloid gold ICS. As well as indirect immunofluorescence, the AOL-conjugated colloid gold ICS showed reliable results in the serum of 39 SLE patients. Our results indicated that the AOL-conjugated colloid gold ICS could serve as a rapid test for the detection of ANA and suspected cases of SLE.
Highlights
Systemic lupus erythematosus (SLE) is a complex multi-system autoimmune disease
We established a rapid and sensitive immunochromatographic strips (ICS) using prokaryotically expressed Aspergillus oryzae lectin (AOL) and C3 domain repeated protein G (SpG3), which is specific for L-fucose recognition of serum I gG20 for the diagnosis of SLE
We detected the level of core fucosylation in the sera of autoimmune diseases (ADs) patients and found that core fucosylation was significantly increased in AD patients, evidenced by lectin blot with AOL, which is specific for L-fucose recognition (*p < 0.05) (Fig. 2A,B)
Summary
Systemic lupus erythematosus (SLE) is a complex multi-system autoimmune disease. Detection of anti-nuclear antibodies (ANA) is fundamental for the diagnosis of SLE. We found that the level of core fucosylation catalyzed by α1,6-fucosyltransferase (Fut8) is markedly up-regulated on immunoglobulin G (IgG) in the sera of SLE patients detected by Aspergillus oryzae lectin (AOL) blot. The detection limit of core fucosylated IgG was 10 μg/mL for AOL-conjugated colloid gold ICS. As well as indirect immunofluorescence, the AOL-conjugated colloid gold ICS showed reliable results in the serum of 39 SLE patients. Our results indicated that the AOL-conjugated colloid gold ICS could serve as a rapid test for the detection of ANA and suspected cases of SLE. Lectin-ELISA using the fucose-lectin, Aleuria aurantia lectin (AAL), could detect the fucosylated N-glycan[7] These tests need more complex and expensive requirements that limit its application to SLE patients. We considered that hyper core fucosylation of IgG may contribute to SLE pathogenesis
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