Abstract
Objective: HPTLC Method for Simultaneous quantification of co-enzyme Q10 and α-tocopherol in bulk and capsule dosage form was developed and validated as per International Conference on Harmonization [(ICH) Q2 (R1)] guideline.Methods: The chromatograms were developed using a mobile phase of Toluene: ethyl acetate: chloroform (10:1:2 v/v/v) on Pre-coated silica 60F 254 plates and quantified by densitometric absorbance mode at 280 nm.Results: The Rf values were 0.77 and 0.87 for co-enzyme Q10 and α-tocopherol, respectively. The linearity of the method was found to be in the concentration range of 0.6µg-1.8 µg/band for α-tocopherol and 2 µg-6 µg/band for co-enzyme Q10. The limits of detection and quantification were 0.3154 and 0.9559 µg/band for α-tocopherol and 3.441 and 10.42 µg/band for co-enzyme Q10.Conclusion: Developed densitometric method was found to be robust, precise, accurate, and rapid and can be used to analyse fixed-dose capsule samples of co-enzyme Q10 and α-tocopherol.
Highlights
HPTLC is a well-known, and versatile separation method which is type of planar chromatography involving the principle of adsorption
Being a part of a family of quinone compounds known as coenzyme Q, co-enzyme Q10 is characterized by a quinone ring attached to a repeating series of side-chain isoprene units
The chromatograms of blank, standard mixture of co-enzyme Q10 and α-tocopherol and Sample mixture of co-enzyme Q10 and α-tocopherol in the formulation are shown in fig. 3, fig. 4 and fig
Summary
HPTLC is a well-known, and versatile separation method which is type of planar chromatography involving the principle of adsorption. HPTLC serves as a preferred analytical tool for quantitative analysis of drug substances in bulk, from their formulations, from the biological matrix, analysis of herbal extracts and standardization of herbal drugs [1]. Co-enzyme Q 10 (co-enzyme Q10; ubidecarenone) is a biologically active compound that is similar in chemical structure to menaquinones (vitamin K2). Being a part of a family of quinone compounds known as coenzyme Q, co-enzyme Q10 is characterized by a quinone ring attached to a repeating series of side-chain isoprene units The number of isoprene units is denoted by the co-enzyme-X designation. In the case of co-enzyme Q10, there are 10 repeating isoprene units [2]
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