Abstract
An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B1, B2, B5, B6, B9, B12) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol. Total run time was 35 minutes. Detection was performed with diode array set at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples (24 plasma and urine samples from abstinent alcohol-dependent males). Interday and intraday precision were <4% and <7%, respectively, for all vitamins. Recovery percentages ranged from 93% to 100%.
Highlights
Water-soluble vitamins include B group vitamins (B1, B2, B3, B5, B6, B9, B12) and ascorbic acid
Vitamins are micronutrients that are essential to life, and many of them play an important role in regulation of brain functioning
A number of recent studies have focused on validation of analytical methodologies for multivitamins analysis but the vast majority of them applied their methods to analysis of food matrices, drinks, polyvitaminated premixes, and vitamins supplements [10, 11]
Summary
Water-soluble vitamins include B group vitamins (B1, B2, B3, B5, B6, B9, B12) and ascorbic acid (vitamin C). Only a relatively small number of experimental studies focused on validation of analytical methodologies for multivitamins analysis in biological samples (blood and urine) and with limited results in terms of lengthy sample preparation steps and method’s robustness and reproducibility [8] Because of this lack of a robust and validated analytical test for multivitamin analysis in biological samples in routine clinical assessment and in those investigations where a timely and robust analytical method is needed, the aim of this study was to develop and validate a novel HPLC methodology for rapid detection and quantitation of seven water-soluble vitamins (B1, B2, B5, B6, B9, B12, C) in biological fluids (plasma and urine). The validated method was applied to quantify watersoluble vitamins in plasma and urine samples obtained from 24 abstinent alcohol-dependent males
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
