A novel Halomonas ventosae-specific virulent halovirus isolated from the Qiaohou salt mine in Yunnan, Southwest China.

Abstract

Although Halomonas phages belonging to the families Myoviridae and Siphoviridae have been reported, no virulent Halomonas siphoviruses are known. In this study, a virulent bacteriophage, QHHSV-1, of the family Siphoviridae that specifically infects H. ventosae QH52-2 was isolated from the Qiaohou salt mine. Restriction analysis indicated that QHHSV-1 is a dsDNA virus with a genome size of 33.5-39.5kb. Transmission electron microscopy showed that QHHSV-1 is a typical representative of the Siphoviridae, with an icosahedral head (47nm in diameter) and a non-contractile tail (75nm in length). We also assessed the adsorption rate of QHHSV-1 for the host bacterium and found significant inhibition after the addition of 10mM CaCl2. Based on a one-step growth curve, we determined a latent period of 30min and a burst size of 73 PFU/infected cell. At the optimal pH of 8.0, 25.9 and 15.2% of the phages survived after a 60-min incubation at 50 and 60°C, respectively. Phage replication was possible at a wide range of salt concentrations, from 2.0 to 20% (w/v), with an optimum concentration of 5%. The survival of QHHSV-1 at different salt concentrations decreased with time and 25% survival after 25days at 30% salt concentration.