Abstract
Although Halomonas phages belonging to the families Myoviridae and Siphoviridae have been reported, no virulent Halomonas siphoviruses are known. In this study, a virulent bacteriophage, QHHSV-1, of the family Siphoviridae that specifically infects H. ventosae QH52-2 was isolated from the Qiaohou salt mine. Restriction analysis indicated that QHHSV-1 is a dsDNA virus with a genome size of 33.5-39.5kb. Transmission electron microscopy showed that QHHSV-1 is a typical representative of the Siphoviridae, with an icosahedral head (47nm in diameter) and a non-contractile tail (75nm in length). We also assessed the adsorption rate of QHHSV-1 for the host bacterium and found significant inhibition after the addition of 10mM CaCl2. Based on a one-step growth curve, we determined a latent period of 30min and a burst size of 73 PFU/infected cell. At the optimal pH of 8.0, 25.9 and 15.2% of the phages survived after a 60-min incubation at 50 and 60°C, respectively. Phage replication was possible at a wide range of salt concentrations, from 2.0 to 20% (w/v), with an optimum concentration of 5%. The survival of QHHSV-1 at different salt concentrations decreased with time and 25% survival after 25days at 30% salt concentration.
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