Abstract
: During the last decades, there has been growing interest in using therapeutic messager RNA (mRNA) together with drug delivery systems. Naked, unformulated mRNA is, however, unable to cross the cell membrane and is susceptible to degradation. Here we use graphene quantum dots (GQDs) functionalized with polyethyleneimine (PEI) as a novel mRNA delivery system. Our results show that these modified GQDs can be used to deliver intact and functional mRNA to Huh‐7 hepatocarcinoma cells at low doses and, that the GQDs are not toxic, although cellular toxicity is a problem for these first‐generation modified particles. Functionalized GQDs represent a potentially interesting delivery system that is easy to manufacture, stable and effective.
Highlights
In recent years, graphene quantum dots (GQDs) have emerged as an attractive platform for bio-applications, involving biosensing, biomedical imaging and drug delivery
Functionalized GQDs (FGQDs) to messager RNA (mRNA) ratios were expressed as molar ratios of N in FGQDs to molar ratios of P in mRNA (N/P ratio)
Our results show that FGQDs were able to successfully complex mRNA and deliver it intact to the cytoplasm of Huh-7 hepatocarcinoma cells
Summary
N.B. When citing this work, cite the original published paper. Research.chalmers.se offers the possibility of retrieving research publications produced at Chalmers University of Technology. It covers all kind of research output: articles, dissertations, conference papers, reports etc. Research.chalmers.se is administrated and maintained by Chalmers Library (article starts on page). Ya Liu+,[a, b] Changhong Zhao+,[a, f] Alan Sabirsh+,[c] Lilei Ye,[d] Xiaoqiu Wu,*[c] Hongbin Lu,*[b] and Johan Liu*[a, e]
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