Abstract
Ag doped CeO2 nanoparticles have been prepared by using green synthesized Ag and CeO2 nanoparticles. The formation of Ag doped CeO2 nanoparticles has been confirmed by SEM and EDAX analysis. Modified graphite Ag doped CeO2 electrode was prepared to study the biosensor using cyclic voltammetry method. The cyclic voltammetry was used as detection method to detect glucose in which the charge under the peak was calculated in a specific potential range. Methanol in PBS buffer (pH=7.0) was used as the flowing eluent. It is a pair of well-reversible redox reagents used as an electron-transfer mediator. Effect of scan rate, solution flow rate and buffer pH were also studied. The results show a small peak current due to the redox waves with formal potential at 1.705 volts. Here glucose oxidase undergoes catalytic oxidation. The current increases and creates the significant amount of electro catalytic current. This is due to the production of H2O2 by glucose oxidase enzyme during the oxidation of glucose.
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