A novel galactose- and arabinose-specific lectin from the sponge Pellina semitubulosa: isolation, characterization and immunobiological properties

Abstract

A new lectin from the sponge Pellina semitubulosa is derived which was extracted and purified to homogeneity. The purified lectin is probably a hexamer of polypeptide chains (each M r 34 000) which are covalently linked via disulfide linkages; the isoelectric point is 6.1. The lectin displays the following specificities: D-galactose ( 50% inhibition of hemagglutination at 0.2 mM = L-arabinose (0.2 mM) > D-fucose (1.5 mM) > D-glucose (3.0 mM) . It precipitates human erythrocytes (A 1, A 2, A 1B, B, and O) with a titer between 2 8 and 2 11 and erythrocytes from sheep and rabbits with a titer between 2 5 and 2 10. The Pellina lectin displays a strong mitogenic effect on spleen lymphocytes from mice. Immunochemical analyses revealed that both murine T- and B-lymphocytes display a capping of the lectin receptors on their cell surfaces after lectin treatment. Murine macrophages were found to endocytose the lectin. Pellina lectin at concentrations between 0.3 and 10.0 μg/ml potently enhances interleukin 1 (IL-1) release from mouse peritoneal macrophages and interleukin 2 (IL-2) production in mixed murine lymphocyte cultures.