Abstract
The aim for the present study was to evaluate the in vitro effects of iMatrix-411 in odontoblast-like cells. To that end, iMatrix-411 was coated to both nontissue culture treated- (Non-PS) and tissue culture treated-polystyrene (TCPS) multiwells. MDPC-23 cells were seeded into noncoated (control) or coated wells. Optimal coating density and cell proliferation were assessed by cell counting kit-8 (CCK-8) at day two, day three, and day five. Osteo/odontogenic differentiation was evaluated by real-time RT-PCR and alkaline phosphatase (ALP) activity at days seven and eight, respectively. Calcific deposition of cells was visualized by alizarin red staining. Data were analyzed with post hoc Tukey HSD test (p < 0.05). Optimal coating density for iMatrix-411 was 8 μg/cm2. Exposure of MDPC-23 cells to iMatrix-411 in either non-PS or TCPS significantly enhanced proliferative activity. iMatrix-411 elevated ALP activity in both types of culture plates. iMatrix-411 significantly increased the mRNA level of OCN, BSP, OPN, ALP, and DMP-1. Meanwhile, it enhanced the expression of several integrin subunits: ITGA1, ITGA5, ITGAV, ITGB1, and ITGB5. Finally, iMatrix-411 also accelerated the mineralization at day eight in Non-PS. The results indicated iMatrix-411 stimulates proliferation and favours differentiation of odontoblast-like cells.
Highlights
Calcium hydroxide (CH) is commonly used for capping exposed or nearly exposed pulp in an effort to initiate a healing process within wound site
To elucidate the influence of a novel fragment derived from LN-411 in odontoblast-like cells, we analyzed in vitro the proliferation and differentiation of MDPC-23 cells to iMatrix-411 and searched for integrin receptors involved
We found that iMatrix-411 promoted the mRNA expression of DMP-1 to a fold change that is similar to alkaline phosphatase (ALP)
Summary
Calcium hydroxide (CH) is commonly used for capping exposed or nearly exposed pulp in an effort to initiate a healing process within wound site. Its nine-year success rate (SR) is only 58.7% [1] and the SR tends to further decrease over time, primarily due to high alkalinity and poor adhesiveness of CH to dentine tissue. When the pulp is exposed for protracted period, the possibility of pulp infection could not be ruled out. Under this circumstance, a more invasive treatment named root canal therapy (RCT) needs to be implemented. RCT sacrifices the sensory and forming ability for structural integrity of teeth. It is of critical importance to maintain the integrity of dentine structure before infection reaches the pulp
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