Abstract
A novel fluorophotometric method for the detection and determination of amino sugars was established utilizing pyridoxal-HCl and Zn(II) ion. This method was highly sensitive; its limit of determination was 1 × 10 −11 mole of amino sugars. The fluorogen of this fluorescence reaction was Zn(II) chelate of N-pyridoxylidene amino sugar containing a 1:1 ratio of amino sugar to Zn(II) ion. The hexosamine content of chondroitin sulfate A and C, hyaluronic acid, and heparin estimated by this method was higher than that obtained by the Elson-Morgan method. Only 2–3 hr of hydrolysis with 2 N HCl was sufficient for the assay of chondroitin sulfate and heparin.
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