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Abstract
Parkinson’s disease (PD) is closely related to the oxidative stress induced by excess hydrogen peroxide (H2O2) in organisms. Developing an efficient method for noninvasive and real-time H2O2 detection is beneficial to investigate the role played by H2O2 in PD. In this work, a novel fluorogenic probe (CBH) for living organisms H2O2 detection has been designed, synthesized and characterized. The emission of CBH in PBS solution is very weak. However, when H2O2 was added, the fluorescence of CBH solution was sharply increased for 12-fold, accompanied by the emission peak blue-shifted from 600 to 530 nm. Moreover, the response of CBH to H2O2 is highly sensitive and selective and is not affected by various ROS/RNS, anions, cations, and amino acids. Based on the good performance of CBH for H2O2 detection, it has been successfully applied to visualizing the H2O2 concentration in living cells, Zebrafish and [Formula: see text]. elegans PD models.
Highlights
As a well-known term for chemical species produced upon incomplete reduction of oxygen, reactive oxygen species (ROS) plays a key role in regulating various physiological functions of living organisms.[1,2] The intrinsic biochemical properties of ROS underlie the mechanisms necessary for the development of living organisms
The etiology of Parkinson's disease (PD) is largely unknown, emerging studies have shown that the excess of ROS such as H2O2 causing oxidative stress inside cells is closely connected to PD.[10,11]
Reaction progress was monitored by thin-layer chromatography (TLC) on pre-coated silica plates (250 m thickness) and spots were visualized by UV light
Summary
As a well-known term for chemical species produced upon incomplete reduction of oxygen, reactive oxygen species (ROS) plays a key role in regulating various physiological functions of living organisms.[1,2] The intrinsic biochemical properties of ROS underlie the mechanisms necessary for the development of living organisms. A novel °uorogenic probe (CBH) for living organisms H2O2 detection have been designed and synthesized. Since CBH displayed good performance in H2O2 detection, it was successfully applied to monitor the H2O2 concentration in living cells, Zebrash and C. elegans PD models
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