A novel fluorescent probe for the detection of peroxynitrite and its application in mice epileptic brain model

Abstract

Herein, a fluorescent probe, GYP, was developed for the detection of ONOO− in KA-induced epileptic brains. In solution, as a ratiometric probe, GYP indicated practical properties including steadiness under wide pH range (3.0–12.0), rapid response (within 20 s), stability over 48 h, high sensitivity (LOD = 0.27 μM) and high selectivity. In living PC12 cells, in spite of the low toxicity, GYP could achieve the time-dependent and dose-dependent imaging of ONOO−, while the generation and elimination were checked by introduction of SIN-1 and NAC, respectively. Further, GYP could cross Blood-Brain Barrier (BBB) rapidly and steadily during the imaging in KA-induced mice epileptic brain model. Thus, this work raised a practical implement for the detection of ONOO− in brain region, which might be helpful for further understanding of the epilepsy mechanism in future.