Abstract
A novel fluorescent probe L based on cyanoacetyl indole derivative was designed and synthesized, and its chemical structure was characterized by spectrum analysis. Probe L displayed good stability, highly selectivity and sensitivity for detection of HPO42− through fluorescence quenching, and other thirteen anions including H2PO4−, NO3−, NO2−, HSO3−, SO32−, SO42−, S2O32−, AcO−, F−, Cl−, Br−, I−, SCN− was basically undisturbed and only occurred weak fluorescence changes. According to titration results, the lowest detection limits of probe L under fluorescence were 5.89 × 10−7 mol/L. Due to the linear fitting of Job's plot, it can be determined that the binding stoichiometric ratio between L and HPO42− is 1:1, and their combination by the action of hydrogen bond. A series of sensor strips based on probe L were made to selectively detect HPO42− in the soil sample, and the results indicated probe L exhibited significant applied potential.
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