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https://doi.org/10.1016/j.talanta.2013.10.004
Copy DOIJournal: Talanta | Publication Date: Oct 9, 2013 |
Citations: 36 |
A novel sensing strategy for specific recognition of hemoglobin has been designed. In the presence of trypsin, hemoglobin was cleaved to release heme which could effectively bind to a FITC-labeled guanine (G)-rich oligonucleotide sequence to form the G-quadruplex/hemin complex. This binding process would lead to fluorescence quenching of FITC via photoinduced electron transfer. Therefore, a fluorescence assay for the determination of hemoglobin could be established. Under the optimized conditions, there was a linear relationship between the fluorescence intensity of probe DNA and the hemoglobin concentration in the range of 5-200 nmol/L, with the limit of detection (LOD) as low as 2 nmol/L.
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